The history and conceptual framework of assays and screens.

ABSTRACT: Since the 16th century, assays and screens have been essential for scientific investigation. However, most methods could be significantly improved, especially in accuracy, scalability, and often lack adequate comparisons to negative controls. There is a lack of consistency in distinguishing assays, in which accuracy is the main goal, from screens, in which scalability is prioritized over accuracy. We dissected and modernized the original definitions of assays and screens based upon recent developments and the conceptual framework of the original definitions. All methods have three components: design/measurement, performance, and interpretation. We propose a model of method development in which reproducible observations become new methods, initially assessed by sensitivity. Further developm

SUBMITTER: Giacoletto CJ 

PROVIDER: S-EPMC10024921 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature