Biofilm formation of two genetically diverse Staphylococcus aureus isolates under beta-lactam antibiotics.

ABSTRACT:

Purpose

Our aim was to evaluate the biofilm formation of 2 genetically diverse Staphylococcus aureus isolates, 10379 and 121940, under different concentrations of beta-lactam antibiotics on biomass content and biofilm viability.

Methods

Biofilm formation and methicillin resistance genes were tested using PCR and multiplex PCR. PCR was combined with bioinformatics analysis to detect multilocal sequence typing (MLST) and SCCmec types, to study the genetical correlation between the tested strains. Then, the crystal violet (CV) test and XTT were used to detect biomass content and biofilm activity. Antibiotic susceptibility was tested using a broth dilution method. According to their specific MIC, different concentrations of beta-lactam antibiotics were used to stud

SUBMITTER: Liang J 

PROVIDER: S-EPMC10025342 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature