IBRAP: integrated benchmarking single-cell RNA-sequencing analytical pipeline.

ABSTRACT: Single-cell ribonucleic acid (RNA)-sequencing (scRNA-seq) is a powerful tool to study cellular heterogeneity. The high dimensional data generated from this technology are complex and require specialized expertise for analysis and interpretation. The core of scRNA-seq data analysis contains several key analytical steps, which include pre-processing, quality control, normalization, dimensionality reduction, integration and clustering. Each step often has many algorithms developed with varied underlying assumptions and implications. With such a diverse choice of tools available, benchmarking analyses have compared their performances and demonstrated that tools operate differentially according to the data types and complexity. Here, we present Integrated Benchmarking scRNA-seq Analytical Pipel

SUBMITTER: Knight CH 

PROVIDER: S-EPMC10025434 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature