Efficient short time pretreatment on lignocellulosic waste using an isolated fungus Trametes sp. W-4 for the enhancement of biogas production.

ABSTRACT: Biological pretreatment to the lignocellulosic waste prior to anaerobic digestion is a popular method to increase biogas production. However, the long time needed for the pretreatment is not suitable to the practical application. A fungus strain, which could produce many kinds of lignocellulosic enzymes including CMCase, FPase, xylanase and laccase, was isolated from the soil of Tibet in this study. The fungus was identified as Trametes sp. W-4 by morphological and molecular characterization. The optimum culture temperature was 30 °C and the optimum nitrogen source was peptone. Under the optimum fermentation condition, the activity of CMCase, FPase, xylanase and laccase could reach 2.73 U/mL, 0.41 U/mL, 0.29 U/mL, and 1.11 U/mL, respectively. The results of pretreatment of Tramet

SUBMITTER: Jin X 

PROVIDER: S-EPMC10025918 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature