A straightforward microfluidic-based approach toward optimizing transduction efficiency of HIV-1-derived lentiviral vectors in BCP-ALL cells

ABSTRACT: Highlights • Lentiviral vectors have a limited half-life, and in order to reach the target, the distance between the target cell and the vector should be minimized.• Microfluidic systems provide a micro-scaled environment in which lentiviral vectors have a high chance of reaching the target cell.• Using microfluidic systems decreases the transduction time.• Compared to standard cell culture plates, microfluidic systems require considerably lower culture media and reagents for a transduction procedure.• Microfluidic system has proven to be very beneficial in enhancing lentiviral transduction.

Background

HIV-1-derived lentiviral vectors (LVs) are capable of transducing human cells by integrating the transgene into the host genome. In order to do that, LVs should have enough time and

SUBMITTER:  

PROVIDER: S-EPMC10025989 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature