Abnormal prion protein, infectivity and neurofilament light-chain in blood of macaques with experimental variant Creutzfeldt-Jakob disease.

ABSTRACT: Transmissible spongiform encephalopathies (TSEs) are fatal neurodegenerative infections. Variant Creutzfeldt-Jakob disease (vCJD) and sporadic CJD (sCJD) are human TSEs that, in rare cases, have been transmitted by human-derived therapeutic products. There is a need for a blood test to detect infected donors, identify infected individuals in families with TSEs and monitor progression of disease in patients, especially during clinical trials. We prepared panels of blood from cynomolgus and rhesus macaques experimentally infected with vCJD, as a surrogate for human blood, to support assay development. We detected abnormal prion protein (PrP^TSE) in those blood samples using the protein misfolding cyclic amplification (PMCA) assay. PrP^TSE first appeared in the blood of pr

SUBMITTER: Yakovleva O 

PROVIDER: S-EPMC10027005 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature