Involvement of GABAergic Interneuron Subtypes in 4-Aminopyridine-Induced Seizure-Like Events in Mouse Entorhinal Cortex in Vitro.

ABSTRACT: Single-unit recordings performed in temporal lobe epilepsy patients and in models of temporal lobe seizures have shown that interneurons are active at focal seizure onset. We performed simultaneous patch-clamp and field potential recordings in entorhinal cortex slices of GAD65 and GAD67 C57BL/6J male mice that express green fluorescent protein in GABAergic neurons to analyze the activity of specific interneuron (IN) subpopulations during acute seizure-like events (SLEs) induced by 4-aminopyridine (4-AP; 100 μm). IN subtypes were identified as parvalbuminergic (IN_PV, n = 17), cholecystokinergic (IN_CCK), n = 13], and somatostatinergic (IN_SOM, n = 15), according to neurophysiological features and single-cell digital PCR. IN_PV an

SUBMITTER: Scalmani P 

PROVIDER: S-EPMC10027059 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature