Measurement of 24-h continuous human CH₄ release in a whole room indirect calorimeter.

ABSTRACT: We describe the technology and validation of a new whole room indirect calorimeter (WRIC) methodology to quantify volume of methane (VCH₄) released from the human body over 24 h concurrently with the assessment of energy expenditure and substrate utilization. The new system extends the assessment of energy metabolism by adding CH₄, a downstream product of microbiome fermentation that could contribute to energy balance. Our new system consists of an established WRIC combined with the addition of off-axis integrated-cavity output spectroscopy (OA-ICOS) to measure CH₄ concentration ([CH₄]). Development, validation, and reliability of the system included environmental experiments to measure the stability of the atmospheric [CH₄], infusing

SUBMITTER: Carnero EA 

PROVIDER: S-EPMC10027086 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature