Geometric engineering of organoid culture for enhanced organogenesis in a dish.

ABSTRACT: Here, we introduce a facile, scalable engineering approach to enable long-term development and maturation of organoids. We have redesigned the configuration of conventional organoid culture to develop a platform that converts single injections of stem cell suspensions to radial arrays of organoids that can be maintained for extended periods without the need for passaging. Using this system, we demonstrate accelerated production of intestinal organoids with significantly enhanced structural and functional maturity, and their continuous development for over 4 weeks. Furthermore, we present a patient-derived organoid model of inflammatory bowel disease (IBD) and its interrogation using single-cell RNA sequencing to demonstrate its ability to reproduce key pathological features of IBD. Finally

SUBMITTER: Park SE 

PROVIDER: S-EPMC10027401 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature