Frozen fresh blood plasma preserves the functionality of native human α₂-macroglobulin.

ABSTRACT: Human α₂-macroglobulin (hα₂M) is a large homotetrameric protein involved in the broad inhibition of endopeptidases. Following cleavage within a bait region, hα₂M undergoes stepwise transitions from its native, expanded, highly flexible, active conformation to an induced, compact, triggered conformation. As a consequence, the peptidase is entrapped by an irreversible Venus flytrap mechanism. Given the importance of hα₂M, biochemical studies galore over more than seven decades have attempted to ascertain its role, typically using authentic hα₂M purified from frozen and non-frozen fresh blood plasma, and even outdated plasma. However, hα₂M is sensitive once isolated and purified, and becomes heterogeneous during storage and/or

SUBMITTER: Mendes SR 

PROVIDER: S-EPMC10027685 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature