Nanopore Sequencing Discloses Compositional Quality of Commercial Probiotic Feed Supplements.

ABSTRACT: The market for the application of probiotics as a livestock health improvement supplement has increased in recent years. However, most of the available products are quality-controlled using low-resolution techniques and un-curated databases, resulting in misidentification and incorrect product labels. In this work, we deployed two workflows and compared results obtained by full-length 16S rRNA genes (16S) and metagenomic (Meta) data to investigate their reliability for the microbial composition of both liquid and solid forms of animal probiotic products using Oxford Nanopore long-read-only (without short-read). Our result revealed that 16S amplicon data permits to detect the bacterial microbiota even with the low abundance in the samples. Moreover, the 16S approach has the potential to pro

SUBMITTER: Kruasuwan W 

PROVIDER: S-EPMC10027865 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature