Loss of CD28 expression associates with severe T-cell exhaustion in acute myeloid leukemia.

ABSTRACT:

Introduction

Despite accumulated evidence in T-cell exhaustion in acute myeloid leukemia (AML), the immunotherapeutic targeting exhausted T cells such as programmed cell death protein 1 (PD-1) blockade in AML failed to achieve satisfying efficacy. Characteristics of exhausted T cells in AML remained to be explored.

Methods

Phenotypic analysis of T cells in bone marrow (BM) using flow cytometry combining senescent and exhausted markers was performed in de novo AML patients and healthy donors as well as AML patients with complete remission (CR). Functional analysis of T-cell subsets was also performed in de novo AML patients using flow cytometry.

Results

T cells experienced a phenotypic shift to terminal differentiation characterized by increased loss of CD28 expression

SUBMITTER: Huang Y 

PROVIDER: S-EPMC10027902 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature