RNA-seq and ATAC-seq analysis of CD163⁺ macrophage-induced progestin-insensitive endometrial cancer cells.

ABSTRACT:

Background

Progestins are used as fertility-sparing regimens for young patients with stage 1A endometrioid endometrial cancer (EEC) and atypical endometrial hyperplasia (AEH). CD163⁺ macrophages promote estrogen-dependent EEC development, but whether they induce progestin insensitivity remains unclear. This study aimed to investigate the possible effects of CD163⁺ macrophages on progestin response in AEH/EEC patients.

Methods

The number of infiltrating CD163⁺ macrophages in progestin-insensitive and -sensitive endometrial lesions was compared. The effects of CD163⁺ macrophages on progestin responses and progesterone receptor (PR) expression in EC cells were evaluated in vitro. ATAC-seq and RNA-seq were combined to identify molecula

SUBMITTER: Wang L 

PROVIDER: S-EPMC10028121 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature