Mycobacterial IHF is a highly dynamic nucleoid-associated protein that assists HupB in organizing chromatin.

ABSTRACT: Nucleoid-associated proteins (NAPs) crucially contribute to organizing bacterial chromatin and regulating gene expression. Among the most highly expressed NAPs are the HU and integration host factor (IHF) proteins, whose functional homologues, HupB and mycobacterial integration host factor (mIHF), are found in mycobacteria. Despite their importance for the pathogenicity and/or survival of tubercle bacilli, the role of these proteins in mycobacterial chromosome organization remains unknown. Here, we used various approaches, including super-resolution microscopy, to perform a comprehensive analysis of the roles of HupB and mIHF in chromosome organization. We report that HupB is a structural agent that maintains chromosome integrity on a local scale, and that the lack of this protein alters c

SUBMITTER: Holowka J 

PROVIDER: S-EPMC10028186 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature