CRISPR/Cas9 editing of the polygalacturonase FaPG1 gene improves strawberry fruit firmness.

ABSTRACT: Firmness is one of the most important fruit quality traits in strawberries. The postharvest shelf life of this soft fruit is highly limited by the loss of firmness, where cell wall disassembly plays an important role. Previous studies demonstrated that the polygalacturonase FaPG1 has a key role in remodelling pectins during strawberry softening. In this study, FaPG1 knockout strawberry plants have been generated using the CRISPR/Cas9 system delivered via Agrobacterium tumefaciens. Ten independent lines, cv. "Chandler", were obtained, and all of them were successfully edited as determined by PCR amplification and T7 endonuclease assay. The targeted mutagenesis insertion and deletion rates were analyzed using targeted deep sequencing. The percentage of edited sequences varied f

SUBMITTER: Lopez-Casado G 

PROVIDER: S-EPMC10028403 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature