Biocatalysis versus Molecular Recognition in Sialoside-Selective Neuraminidase Biosensing.

ABSTRACT: Sialic acid recognition and hydrolysis are essential parts of cellular function and pathogen infectivity. Neuraminidases are enzymes that detach sialic acid from sialosides, and their inhibition is a prime target for viral infection treatment. The connectivity and type of sialic acid influence the recognition and hydrolysis activity of the many different neuraminidases. The common strategies to evaluate neuraminidase activity, recognition, and inhibition rely on extensive labeling and require a large amount of sialylated glycans. The above limitations make the effort of finding viral inhibitors extremely difficult. We used synthetic sialylated glycans and developed a label-free electrochemical method to show that sialoside structural features lead to selective neuraminidase biosensing. We

SUBMITTER: Alshanski I 

PROVIDER: S-EPMC10028605 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature