Pharmacological characterization of the endocannabinoid sensor GRAB_eCB2.0.

ABSTRACT:

Introduction

The endocannabinoids (eCBs), 2-arachidonoylglycerol (2-AG) and arachidonoyl ethanolamine (AEA), are produced by separate enzymatic pathways, activate cannabinoid receptors with distinct pharmacology, and differentially regulate pathophysiological processes. The genetically encoded sensor, GRAB_eCB2.0, detects real-time changes in eCB levels in cells in culture and preclinical model systems; however, its activation by eCB analogues produced by cells and by phyto-cannabinoids remains uncharacterized, a current limitation when interpreting changes in its response. This information could provide additional utility for the tool in in vivo pharmacology studies of phyto-cannabinoid action.

Methods

GRAB_eCB2.0 was expressed in cu

SUBMITTER: Singh S 

PROVIDER: S-EPMC10028790 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature