The cell type composition of the adult mouse brain revealed by single cell and spatial genomics.

ABSTRACT: The function of the mammalian brain relies upon the specification and spatial positioning of diversely specialized cell types. Yet, the molecular identities of the cell types, and their positions within individual anatomical structures, remain incompletely known. To construct a comprehensive atlas of cell types in each brain structure, we paired high-throughput single-nucleus RNA-seq with Slide-seq-a recently developed spatial transcriptomics method with near-cellular resolution-across the entire mouse brain. Integration of these datasets revealed the cell type composition of each neuroanatomical structure. Cell type diversity was found to be remarkably high in the midbrain, hindbrain, and hypothalamus, with most clusters requiring a combination of at least three discrete gene expression m

SUBMITTER: Langlieb J 

PROVIDER: S-EPMC10028805 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature