RNA Sensing and Innate Immunity Constitutes a Barrier for Interspecies Chimerism.

ABSTRACT: Interspecies chimera formation with human pluripotent stem cells (PSCs) holds great promise to generate humanized animal models and provide donor organs for transplant. However, the approach is currently limited by low levels of human cells ultimately represented in chimeric embryos. Different strategies have been developed to improve chimerism by genetically editing donor human PSCs. To date, however, it remains unexplored if human chimerism can be enhanced in animals through modifying the host embryos. Leveraging the interspecies PSC competition model, here we discovered retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) signaling, an RNA sensor, in "winner" cells plays an important role in the competitive interactions between co-cultured mouse and human PSCs. We found that genet

SUBMITTER: Hu Y 

PROVIDER: S-EPMC10028900 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature