Tracking live-cell single-molecule dynamics enables measurements of heterochromatinassociated protein-protein interactions.

ABSTRACT: Visualizing and measuring molecular-scale interactions in living cells represents a major challenge, but recent advances in microscopy are bringing us closer to achieving this goal. Single-molecule super-resolution microscopy enables high-resolution and sensitive imaging of the positions and movement of molecules in living cells. HP1 proteins are important regulators of gene expression because they selectively bind and recognize H3K9 methylated (H3K9me) histones to form heterochromatin-associated protein complexes that silence gene expression. Here, we extended live-cell single-molecule tracking studies in fission yeast to determine how HP1 proteins interact with their binding partners in the nucleus. We measured how genetic perturbations that affect H3K9me alter the diffusive properties o

SUBMITTER: Chen Z 

PROVIDER: S-EPMC10028927 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature