High fidelity sensory-evoked responses in neocortex after intravenous injection of genetically encoded calcium sensors.

ABSTRACT: Two-photon imaging of genetically-encoded calcium indicators (GECIs) has traditionally relied on intracranial injections of adeno-associated virus (AAV) or transgenic animals to achieve expression. Intracranial injections require an invasive surgery and result in a relatively small volume of tissue labeling. Transgenic animals, although they can have brain-wide GECI expression, often express GECIs in only a small subset of neurons, may have abnormal behavioral phenotypes, and are currently limited to older generations of GECIs. Inspired by recent developments in the synthesis of AAVs that readily cross the blood brain barrier, we tested whether an alternative strategy of intravenously injecting AAV-PhP.eB is suitable for two-photon calcium imaging of neurons over many months after injectio

SUBMITTER: Leikvoll A 

PROVIDER: S-EPMC10028972 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature