An animal-free preclinical drug screening platform based on human precision-cut kidney slices.

ABSTRACT:

Objective

Renal fibrosis is one of the main pathophysiological processes underlying the progression of chronic kidney disease and kidney allograft failure. In the past decades, overwhelming efforts have been undertaken to find druggable targets for the treatment of renal fibrosis, mainly using cell- and animal models. However, the latter often do not adequately reflect human pathogenesis, obtained results differ per strain within a given species, and the models are associated with considerable discomfort for the animals. Therefore, the objective of this study is to implement the 3Rs in renal fibrosis research by establishing an animal-free drug screening platform for renal fibrosis based on human precision-cut kidney slices (PCKS) and by limiting the use of reagents that are associ

SUBMITTER: Mutsaers HAM 

PROVIDER: S-EPMC10029185 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature