Mitochondrial DNA D-loop hyper-variable region 1 variability in Kurdish horse breed.

ABSTRACT:

Background

Kurdish horse is one of the most valuable horse genetic resources in the Middle East.

Objectives

To assess the genetic diversity of Kurdish horses, Mitochondrial DNA D-loop hyper-variable region1 (HVR1) was sequenced in 29 non-related Kurdish horses which were sampled from diverse geographic regions of Iran.

Methods

Total DNA was extracted from the collected blood samples by modified salting out method. The HVR1 was amplified by PCR and then sequenced using ABI PRISM BigDyeTM Terminator Cycle Sequencing Ready Reaction Kit. Consequently, the sequences were trimmed to 294 bp using BIOEDIT to become comparable with other reported HVR1 sequences in GeneBank. Sequence alignment was performed using CLUSTALW package. Haplotype and nucleotide diversity were estimat

SUBMITTER: Nikbakhsh M 

PROVIDER: S-EPMC10029883 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature