Assessment of genetic markers for multilocus sequence typing (MLST) of Fasciola isolates from Iran.

ABSTRACT:

Background

Several markers have been described to characterise the population structure and genetic diversity of Fasciola species (Fasciola hepatica (F. hepatica) and Fasciola gigantica (F. gigantica). However, sequence analysis of a single genomic locus cannot provide sufficient resolution for the genetic diversity of the Fasciola parasite whose genomes are ∼1.3 GB in size.

Objectives

To gain a better understanding of the gene diversity of Fasciola isolates from western Iran and to identify the most informative markers as candidates for epidemiological studies, five housekeeping genes were evaluated using a multilocus sequence typing (MLST) approach.

Methods

MLST analysis was developed based on five genes (ND1, Pepck, Pold, Cyt b and HSP70) after genomic DNA extracti

SUBMITTER: Nazari N 

PROVIDER: S-EPMC10029898 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature