A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution.

ABSTRACT: Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of Drosophila neuroscience. Neuronal targeting is typically documented using light microscopy of full GAL4 expression patterns, which generally lack the single-cell resolution required for reliable cell type identification. Here, we use stochastic GAL4 labeling with the MultiColor FlpOut approach to generate cellular resolution confocal images at large scale. We are releasing aligned images of 74,000 such adult central nervous systems. An anticipated use of this resource is to bridge the gap between neurons identified by electron or light microscopy. Identifying individual neurons that make up each GAL4 expression pattern improves the prediction of split-GAL4 combinations targe

SUBMITTER: Meissner GW 

PROVIDER: S-EPMC10030108 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature