Identification of cell culture contamination by an unusual species of Mycoplasma related to the M. mycoides cluster.

ABSTRACT: Mycoplasma contamination is a significant problem in cell culture replication and maintenance. From more than 200 known species, a limited number of Mycoplasma species have been detected in cell cultures, representing new species or variants that can escape detection systems. A qPCR commercial kit was used for Mycoplasma detection in cell cultures. Furthermore, an amplified Mycoplasma species was sequenced and summited for sequence assembly, clustering, and evolutionary analysis study. Our work has identified a new and unusual variant or species of Mycoplasma that possesses a high degree of homology with species related with M. mycoides cluster. This variant is usually associated with cattle but has been detected contaminating a cell culture. My

SUBMITTER: Carrillo-Avila JA 

PROVIDER: S-EPMC10030751 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature