Multiplex translaminar imaging in the spinal cord of behaving mice

ABSTRACT: While the spinal cord is known to play critical roles in sensorimotor processing, including pain-related signaling, corresponding activity patterns in genetically defined cell types across spinal laminae have remained challenging to investigate. Calcium imaging has enabled cellular activity measurements in behaving rodents but is currently limited to superficial regions. Here, using chronically implanted microprisms, we imaged sensory and motor-evoked activity in regions and at speeds inaccessible by other high-resolution imaging techniques. To enable translaminar imaging in freely behaving animals through implanted microprisms, we additionally developed wearable microscopes with custom-compound microlenses. This system addresses multiple challenges of previous wearable microscopes, includ

SUBMITTER: Shekhtmeyster P 

PROVIDER: S-EPMC10030868 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature