EtcPRS ^Mut as a molecular marker of halofuginone resistance in Eimeria tenella and Toxoplasma gondii.

ABSTRACT: The control of coccidiosis, causing huge economic losses in the poultry industry, is facing the stagnation of the development of new drugs and the emergence of drug resistance. Thus, the priority for coccidiosis control is to decipher the effect mechanisms and resistance mechanisms of anticoccidial drugs. In this study, we mined and validated a molecular marker for halofuginone resistance in Eimeria tenella through forward and reverse genetic approaches. We screened whole-genome sequencing data and detected point mutations in the ETH2_1020900 gene (encoding prolyl-tRNA synthetase, PRS). Then, we introduced this mutated gene into E. tenella and Toxoplasma gondii and validated that overexpression of this mutated gene confers resistance to halofuginone in vivo and

SUBMITTER: Sun P 

PROVIDER: S-EPMC10031133 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature