Genome-wide methylation analysis of circulating tumor DNA: A new biomarker for recurrent glioblastom.

ABSTRACT:

Background

Glioblastoma (GBM) is a malignant tumor with a short survival and poor prognosis and a lack of clinically validated biomarkers for diagnosis and prognosis.

Methods

We collected cerebrospinal fluid (CSF) samples and normal CSF sample from recurrent GBM patients and paired tissue samples. Methylation profiles of CSF circulating tumor DNA (ctDNA) and transcriptional profiles of tumor tissues were analyzed. The China Glioma Genome Atlas (CGGA) database and Gene Expression Omnibus (GEO) was used for data analysis.

Results

Lasso analysis and multiplex Cox analysis were performed using intersecting genes of differentially methylated regions and differentially expressed genes. 8 hub genes were screened to construct diagnostic and prognostic models. Based on these 8

SUBMITTER: Dai L 

PROVIDER: S-EPMC10031355 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature