A D-cysteine desulfhydrase, SlDCD2, participates in tomato fruit ripening by modulating ROS homoeostasis and ethylene biosynthesis.

ABSTRACT: Hydrogen sulfide (H₂S) is involved in multiple processes during plant growth and development. D-cysteine desulfhydrase (DCD) can produce H₂S with D-cysteine as the substrate; however, the potential developmental roles of DCD have not been explored during the tomato lifecycle. In the present study, SlDCD2 showed increasing expression during fruit ripening. Compared with the control fruits, the silencing of SlDCD2 by pTRV2-SlDCD2 accelerated fruit ripening. A SlDCD2 gene-edited mutant was constructed by CRISPR/Cas9 transformation, and the mutant exhibited accelerated fruit ripening, decreased H₂S release, higher total cysteine and ethylene contents, enhanced chlorophyll degradation and increased carotenoid accumulation. Additionall

SUBMITTER: Zhao YQ 

PROVIDER: S-EPMC10031741 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature