Systematic dissection, preservation, and multiomics in whole human and bovine hearts.

ABSTRACT:

Objectives

We sought to develop a rigorous, systematic protocol for the dissection and preservation of human hearts for biobanking that expands previous success in postmortem transcriptomics to multiomics from paired tissue.

Background

Existing cardiac biobanks consist largely of biopsy tissue or explanted hearts in select diseases and are insufficient for correlating whole organ phenotype with clinical data.

Methods

We demonstrate optimal conditions for multiomics interrogation (ribonucleic acid (RNA) sequencing, untargeted metabolomics) in hearts by evaluating the effect of technical variables (storage solution, temperature) and simulated postmortem interval (PMI) on RNA and metabolite stability. We used bovine (n=3) and human (n=2) hearts fixed in PAXgene or snap-f

SUBMITTER: Moreira JD 

PROVIDER: S-EPMC10031913 | biostudies-literature | 2023 Mar-Apr

REPOSITORIES: biostudies-literature