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Exploiting Endogenous Enzymes for Cancer-Cell Selective Metabolic Labeling of RNA in Vivo.

ABSTRACT: Tissues and organs are composed of many diverse cell types, making cell-specific gene expression profiling a major challenge. Herein we report that endogenous enzymes, unique to a cell of interest, can be utilized to enable cell-specific metabolic labeling of RNA. We demonstrate that appropriately designed "caged" nucleosides can be rendered active by serving as a substrate for cancer-cell specific enzymes to enable RNA metabolic labeling, only in cancer cells. We envision that the ease and high stringency of our approach will enable expression analysis of tumor cells in complex environments.

SUBMITTER: Beasley S

PROVIDER: S-EPMC10032647 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

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Exploiting Endogenous Enzymes for Cancer-Cell Selective Metabolic Labeling of RNA in Vivo.

Beasley Samantha S Vandewalle Abigail A Singha Monika M Nguyen Kim K England Whitney W Tarapore Eric E Dai Nan N Corrêa Ivan R IR Atwood Scott X SX Spitale Robert C RC

Journal of the American Chemical Society 20220413 16

Tissues and organs are composed of many diverse cell types, making cell-specific gene expression profiling a major challenge. Herein we report that endogenous enzymes, unique to a cell of interest, can be utilized to enable cell-specific metabolic labeling of RNA. We demonstrate that appropriately designed "caged" nucleosides can be rendered active by serving as a substrate for cancer-cell specific enzymes to enable RNA metabolic labeling, only in cancer cells. We envision that the ease and high ...[more]

PMID: 35416650

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