Different-sized extracellular vesicles derived from stored red blood cells package diverse cargoes and cause distinct cellular effects.

ABSTRACT:

Background

The formation of extracellular vesicles (EVs) occurs during cold storage of RBCs. Transfusion of EVs may contribute to adverse responses in recipients receiving RBCs. However, EVs are poorly characterized with limited data on whether distinct vesicles are formed, their composition, and potential biological effects.

Study design and methods

Stored RBC-derived EVs were purified using protocols that separate larger microvesicle-like EVs (LEVs) from smaller exosome-like vesicles (SEVs). Vesicles were analyzed by electron microscopy, content of hemoglobin, heme, and proteins (by mass spectrometry), and the potential to mediate lipid peroxidation and endothelial cell permeability in vitro.

Results

SEVs were characterized by having an electron-dense double membran

SUBMITTER: Oh JY 

PROVIDER: S-EPMC10033430 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature