Bacterial extracellular vesicles in the microbiome of first-pass meconium in newborn infants.

ABSTRACT:

Background

Bacterial extracellular vesicles (EVs) are more likely to cross biological barriers than whole-cell bacteria. We previously observed EV-sized particles by electron microscopy in the first-pass meconium of newborn infants. We hypothesized that EVs may be of bacterial origin and represent a novel entity in the human microbiome during fetal and perinatal periods.

Methods

We extracted EVs from first-pass meconium samples of 17 newborn infants and performed bacterial 16S rRNA gene sequencing of the vesicles. We compared the EV content from the meconium samples of infants based on the delivery mode, and in vaginal delivery samples, based on the usage of intrapartum antibiotics.

Results

We found bacterial EVs in all first-pass meconium samples. All EV samples had

SUBMITTER: Turunen J 

PROVIDER: S-EPMC10033452 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature