Development of high-copy number plasmids in Pseudoalteromonas haloplanktis TAC125.

ABSTRACT: The Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 (PhTAC125) is considered an interesting alternative host for the recombinant protein production, that can be explored when the conventional bacterial expression systems fail. Indeed, the manufacture of all the difficult-to-express proteins produced so far in this bacterial platform gave back soluble and active products. Despite these promising results, the low yield of recombinant protein production achieved is hampering the wider and industrial exploitation of this psychrophilic cell factory. All the expression plasmids developed so far in PhTAC125 are based on the origin of replication of the endogenous pMtBL plasmid and are maintained at a very low copy number. In this work, we set up an experimental strategy to select mutate

SUBMITTER: Calvanese M 

PROVIDER: S-EPMC10033558 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature