New AAV tools fail to detect Neurod1-mediated neuronal conversion of Muller glia and astrocytes in vivo.

ABSTRACT:

Background

Reprogramming resident glial cells to convert them into neurons in vivo represents a potential therapeutic strategy that could replenish lost neurons, repair damaged neural circuits, and restore function. AAV (adeno-associated virus)-based expression systems are powerful tools for in vivo gene delivery in glia-to-neuron reprogramming, however, recent studies show that AAV-based gene delivery of Neurod1 into the mouse brain can cause severe leaky expression into endogenous neurons leading to misinterpretation of glia-to-neuron conversion.

Methods

AAV-based delivery systems were modified for improved in vivo delivery of Neurod1, Math5, Ascl1, and Neurog2 in the adult mouse retina and brain. To examine whether bona fide glia-to-neuron conversion occurs, stringent fat

SUBMITTER: Xie Y 

PROVIDER: S-EPMC10033723 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature