MicroRNA-501 controls myogenin⁺/CD74⁺ myogenic progenitor cells during muscle regeneration.

ABSTRACT:

Objective

Skeletal muscle regeneration is markedly impaired during aging. How adult muscle stem cells contribute to this decrease in regenerative capacity is incompletely understood. We investigated mechanisms of age-related changes in myogenic progenitor cells using the tissue-specific microRNA 501.

Methods

Young and old C57Bl/6 mice were used (3 months or 24 months of age, respectively) with or without global or tissue-specific genetic deletion of miR-501. Muscle regeneration was induced using intramuscular cardiotoxin injection or treadmill exercise and analysed using single cell and bulk RNA sequencing, qRT-PCR and immunofluorescence. Muscle fiber damage was assessed with Evan`s blue dye (EBD). In vitro analysis was performed in primary muscle cells obtained from mice an

SUBMITTER: Fahrner A 

PROVIDER: S-EPMC10033748 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature