BNIP3-mediated autophagy via the mTOR/ULK1 pathway induces primordial follicle loss after ovarian tissue transplantation.

ABSTRACT:

Purpose

To explore the underlying mechanism of primordial follicle loss in the early period following ovarian tissue transplantation (OTT).

Methods

BNIP3 was selected through bioinformatic protocols, as the hub gene related to autophagy during OTT. BNIP3 and autophagy in mice ovarian grafts and in hypoxia-mimicking KGN cells were detected using immunohistochemistry, transmission electron microscopy (TEM), western blotting, qPCR, and fluorescence staining. The regulatory role played by BNIP3 overexpression and the silencing of KGN cells in autophagy via the mTOR/ULK1 pathway was investigated.

Results

Ultrastructure examination showed that autophagic vacuoles increased after mice ovarian auto-transplantation. The BNIP3 and autophagy-related proteins (Beclin-1, LC3B, and

SUBMITTER: Liu F 

PROVIDER: S-EPMC10033815 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature