A matrigel-free method for culture of pancreatic endocrine-like cells in defined protein-based hydrogels.

ABSTRACT: The transplantation of pancreatic endocrine islet cells from cadaveric donors is a promising treatment for type 1 diabetes (T1D), which is a chronic autoimmune disease that affects approximately nine million people worldwide. However, the demand for donor islets outstrips supply. This problem could be solved by differentiating stem and progenitor cells to islet cells. However, many current culture methods used to coax stem and progenitor cells to differentiate into pancreatic endocrine islet cells require Matrigel, a matrix composed of many extracellular matrix (ECM) proteins secreted from a mouse sarcoma cell line. The undefined nature of Matrigel makes it difficult to determine which factors drive stem and progenitor cell differentiation and maturation. Additionally, it is difficult to c

SUBMITTER: Kozlowski MT 

PROVIDER: S-EPMC10033864 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature