Pericytes modulate islet immune cells and insulin secretion through Interleukin-33 production in mice.

ABSTRACT:

Introduction

Immune cells were recently shown to support β-cells and insulin secretion. However, little is known about how islet immune cells are regulated to maintain glucose homeostasis. Administration of various cytokines, including Interleukin-33 (IL-33), was shown to influence β-cell function. However, the role of endogenous, locally produced IL-33 in pancreatic function remains unknown. Here, we show that IL-33, produced by pancreatic pericytes, is required for glucose homeostasis.

Methods

To characterize pancreatic IL-33 production, we employed gene expression, flow cytometry, and immunofluorescence analyses. To define the role of this cytokine, we employed transgenic mouse systems to delete the Il33 gene selectively in pancreatic pericytes, in combination with

SUBMITTER: Burganova G 

PROVIDER: S-EPMC10034381 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature