Separation and single-cell analysis for free gastric cancer cells in ascites and peritoneal lavages based on microfluidic chips.

ABSTRACT:

Backgrounds

Detecting free cancer cells from ascites and peritoneal lavages is crucial for diagnosing gastric cancer (GC). However, traditional methods are limited for early-stage diagnosis due to their low sensitivity.

Methods

A label-free, rapid, and high-throughput technique was developed for separating cancer cells from ascites and peritoneal lavages using an integrated microfluidic device, taking advantage of dean flow fractionation and deterministic lateral displacement. Afterward, separated cells were analyzed using a microfluidic single-cell trapping array chip (SCTA-chip). In situ immunofluorescence for EpCAM, YAP-1, HER-2, CD45 molecular expressions, and Wright-Giemsa staining were performed for cells in SCTA-chips. At last, YAP1 and HER-2 expression in tissues was

SUBMITTER: Zhao J 

PROVIDER: S-EPMC10034419 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature