Oral biofilm dysbiosis during experimental periodontitis.

ABSTRACT:

Objectives

We have previously characterized the main osteoimmunological events that occur during ligature periodontitis. This study aims to determine the polymicrobial community shifts that occur during disease development.

Methods

Periodontitis was induced in C57BL/6 mice using the ligature-induced periodontitis model. Healthy oral mucosa swabs and ligatures were collected every 3 days from 0 to 18 days post-ligature placement. Biofilm samples were evaluated by 16SrRNA gene sequencing (Illumina MiSeq) and QIIME. Time-course changes were determined by relative abundance, diversity, and rank analyses (PERMANOVA, Bonferroni-adjusted).

Results

Microbial differences between health and periodontal inflammation were observed at all phylogenic levels. An evident microbial co

SUBMITTER: Ribeiro AA 

PROVIDER: S-EPMC10034670 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature