Neurotensin stimulates the sperm acrosome reaction and reduces percentages of fertilization in vitro.

ABSTRACT:

Objective

To determine the impact of neurotensin (NTS), a naturally occurring peptide, on the function of human and nonhuman primate sperm.

Design

Experimental study.

Setting

University-based research laboratory.

Patient(s)/animal(s)

Consenting normozoospermic human donors and cynomolgus macaques.

Intervention(s)

None.

Main outcome measure(s)

Sperm acrosome status was assessed. Computer-assisted semen analysis assessed sperm motility, progression, and velocity. Immunocytochemistry and receptor selective agonists were used to identify specific NTS receptors on sperm. Monkey oocytes were obtained after ovarian stimulation, and NTS-treated monkey sperm were used for in vitro fertilization.

Results

Neurotensin treatment of human sperm stimulate

SUBMITTER: Campbell GE 

PROVIDER: S-EPMC10034862 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature