Bivalent GAD autoantibody ELISA improves clinical utility and risk prediction for adult autoimmune diabetes.

ABSTRACT:

Aim/introduction

To investigate the differences in the clinical significance and glutamic acid decarboxylase autoantibody (GADA) affinity between RIA (RIA-GADA) and ELISA (ELISA-GADA) in patients with type 1 diabetes.

Methods

A total of 415 patients with type 1 diabetes were enrolled, including 199 acute-onset type 1 diabetes, 168 slowly progressive type 1 diabetes (SPIDDM), and 48 fulminant type 1 diabetes. GADA affinity was measured by a competitive binding experiment using unlabeled recombinant human GAD65 protein, and the diagnostic performance of both assays and the relationship between GADA affinity and the decline of fasting C-peptide (F-CPR) were examined.

Results

While the ELISA-GADA displayed a higher sensitivity than the RIA method in diagnosing type 1 diab

SUBMITTER: Kawasaki E 

PROVIDER: S-EPMC10034953 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature