Split-marker-mediated genome editing improves homologous recombination frequency in the CTG clade yeast Candida intermedia.

ABSTRACT: Genome-editing toolboxes are essential for the exploration and exploitation of nonconventional yeast species as cell factories, as they facilitate both genome studies and metabolic engineering. The nonconventional yeast Candida intermedia is a biotechnologically interesting species due to its capacity to convert a wide range of carbon sources, including xylose and lactose found in forestry and dairy industry waste and side-streams, into added-value products. However, possibilities of genetic manipulation have so far been limited due to lack of molecular tools for this species. We describe here the development of a genome editing method for C. intermedia, based on electroporation and gene deletion cassettes containing the Candida albicans NAT1 dominant selection marker flanked by 1000 base

SUBMITTER: Peri KVR 

PROVIDER: S-EPMC10035504 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature