The Mobility of Neurofilaments in Mature Myelinated Axons of Adult Mice.

ABSTRACT: Studies in cultured neurons have shown that neurofilaments are cargoes of axonal transport that move rapidly but intermittently along microtubule tracks. However, the extent to which axonal neurofilaments move in vivo has been controversial. Some researchers have proposed that most axonally transported neurofilaments are deposited into a persistently stationary network and that only a small proportion of axonal neurofilaments are transported in mature axons. Here we use the fluorescence photoactivation pulse-escape technique to test this hypothesis in intact peripheral nerves of adult male hThy1-paGFP-NFM mice, which express low levels of mouse neurofilament protein M tagged with photoactivatable GFP. Neurofilaments were photoactivated in short segments of large, myelinated a

SUBMITTER: Fenn JD 

PROVIDER: S-EPMC10035772 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature