SARS-CoV-2 molecular testing and whole genome sequencing following RNA recovery from used BinaxNOW COVID-19 antigen self tests.
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ABSTRACT:
SUBMITTER: Nguyen PV
PROVIDER: S-EPMC10036152 | biostudies-literature | 2023 May
REPOSITORIES: biostudies-literature
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SARS-CoV-2 molecular testing and whole genome sequencing following RNA recovery from used BinaxNOW COVID-19 antigen self tests.
Nguyen Phuong-Vi PV Carmola Ludy Registre LR Wang Ethan E Bassit Leda L Rao Anuradha A Greenleaf Morgan M Sullivan Julie A JA Martin Greg S GS Lam Wilbur A WA Waggoner Jesse J JJ Piantadosi Anne A
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology 20230324
Widespread use of over-the-counter rapid diagnostic tests for SARS-CoV-2 has led to a decrease in availability of clinical samples for viral genomic surveillance. As an alternative sample source, we evaluated RNA isolated from BinaxNOW swabs stored at ambient temperature for SARS-CoV-2 rRT-PCR and full viral genome sequencing. 81 of 103 samples (78.6%) yielded detectable RNA, and 46 of 57 samples (80.7 %) yielded complete genome sequences. Our results illustrate that SARS-CoV-2 RNA extracted fro ...[more]
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