Survival of Escherichia coli after high-antibiotic stress is dependent on both the pregrown physiological state and incubation conditions.

ABSTRACT:

Introduction

The survival of bacterial cells exposed to antibiotics depends on the mode of action, the antibiotics concentration, and the duration of treatment. However, it also depends on the physiological state of the cells and the environmental conditions. In addition, bacterial cultures contain sub-populations that can survive high antibiotic concentrations, so-called persisters. Research on persisters is challenging due to multiple mechanisms for their formation and low fractions, down to and below one millionth of the total cell population. Here, we present an improved version of the persister assay used to enumerate the amount of persisters in a cell population.

Methods

The persister assay with high antibiotic stress exposure was performed at both growth supporting an

SUBMITTER: Thorfinnsdottir LB 

PROVIDER: S-EPMC10036391 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature