Confocal LiDAR for remote high-resolution imaging of auto-fluorescence in aquatic media.

ABSTRACT: Spatially resolved in situ monitoring of plankton can provide insights on the impacts of climate change on aquatic ecosystems due to their vital role in the biological carbon pump. However, high-resolution underwater imaging is technically complex and restricted to small close-range volumes with current techniques. Here, we report a novel inelastic scanning confocal light detection and ranging (LiDAR) system for remote underwater volumetric imaging of fluorescent objects. A continuous wave excitation beam is combined with a pinhole in a conjugated detection plane to reject out-of-focus scattering and accomplish near-diffraction limited probe volumes. The combination of bi-directional scanning with remote focusing enables the acquisition of three-dimensional data. We experimentally determin

SUBMITTER: Santos J 

PROVIDER: S-EPMC10036608 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature