Optimization of extraction conditions of gelatin from buffalo (Bubalus bubalis) skins using response surface methodology.

ABSTRACT: The present study was to determine optimum conditions for gelatin extraction from the skin of buffalo (Bubalus bubalis) using response surface methodology. A central composite design (CCD) was performed to evaluate the effects of NaOH concentration ( X1 ), pre-treatment time ( X2 ), extraction temperature ( X3 ), and extraction time ( X4 ) on the yield (Y ₁), gel strength (Y ₂), and hydroxyproline content (Y ₃) of the extracted gelatin. The optimal combination of the independent variables for a good gelatin yield with high gel strength and hydroxyproline content was found at X1 (0.77 M), X2 (5.08 h), X3 (62.93 °C) and X4 (11.62 h). The experimental values for Y ₁ (16.91%), Y ₂ (236.5 g), and

SUBMITTER: Ramli RA 

PROVIDER: S-EPMC10036889 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature